Array Tomography Movies

Array Tomography of Transgenic Mouse Somatosensory Cortex Layer 5B


Download 1024x768 Pixel Video (55 MB):

C195HD_1024.mov


Reference: Micheva, K.D., and Smith, S.J (2007)  Array tomography: A new tool for imaging the molecular architecture and ultrastructure of neural circuits.  Neuron 55:25-36.


Green: Thy1-H-YFP (Layer 5B Neuron Subset); Red: Synapsin I (Synapses); Blue: DAPI (DNA in all nuclei).

Array Tomography of Amyloid Plaque in Cortex of Alzheimer Mouse


Download 1022x716 Pixel Video (43 MB):

Mouse Plaque 2 1022.mov


Reference: Koffie, R.M., Meyer-Luehmann, M., Hashimoto, T., Adams, K.W., Mielke, M.L., Garcia-Alloza, M., Micheva, K.D., Smith, S.J, Kim, M.L., Lee, V.M., Hyman, B.T., and Spires-Jones, T.L. (2009) Oligomeric amyloid beta associates with postsynaptic densities and correlates with excitatory synapse loss near senile plaques.  Proc. Natl. Acad. Sci., USA 106: 4012-7.

Array Tomography of Amyloid Plaque in Cortex of Deceased Alzheimer Human


Download 1024x768 Pixel Video (53 MB):

Human_AD_Plaque.mov


Reference: Work in progress - Nancy O’Rourke (Stanford), Virginia Lee and John Trojanowski (U. Penn.).


Red: Synapsin I; Green: Amyloid Beta; Blue: Tubulin; Pink: phospho-Tau; Gold: Thio-S staining; White: DAPI DNA.

Array Tomography of Transgenic Mouse Somatosensory Cortex Layer 5A


Download 1024x768 Pixel Video (93.2 MB):

mMos3_YRock8_L5b_YFP_1024_sH264ah.mov


Reference: Micheva, K.D., Busse, B.L., Weiler, N.C., O'Rourke, N., Smith, S.J (2010) Single-synapse analysis of a diverse synapse population: Proteomic imaging methods and markers.  Neuron 68:639-653.


Green: Thy1-H-YFP (Layer 5B Neuron Subset).  Larger spiny branches are dendrites; Very fine crossing filaments are axons.

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Machinery of Mind

(A 5-minute flight through mouse cortex)


QuickTime Downloads:

Machinery_of_Mind_SD.mov (1024x768, 694 MB)

Machinery_of_Mind_HD.mov (1920x1080, 1.24 GB)

Reference:  Micheva, K.D., Busse, B.L., Weiler, N.C., O'Rourke, N., Smith, S.J (2010) Neuron 68:639-653.


Green: Thy1-H-YFP (Layer 5B Neuron Subset); Red: Synapsin I (Synapses); Blue: Tubulin (Dendrite, Axon Cores)

Musical Score: Catherine Rose Smith

(http://www.reverbnation.com/catherinerosesmith)