Array Tomography Technology
Download Neuron technique paper
Download Micheva's detailed protocol
Array tomography allows quantitative imaging of previously unresolvable three-dimensional features of tissue molecular architecture . This new imaging technique is based on building, staining and automated imaging arrays of large numbers of ultrathin serial sections cut from tissue embedded in a hydrophilic resin matrix. Using immunofluorescence microscopy, the result is highly quantitative and extremely detailed three-dimensional images of previously unobservable features of tissue molecular architecture. Ten or more antibodies can be multiplexed on one given array. After immunofluorescence, that array can be imaged again in a scanning electron microscope to acquire voxel-registered ultrastructural information.