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Two-headed Two-Photon Microscope in Stella Coates Neural Imaging Laboratory at Stanford, circa 2002. The output beam from an ultrafast Ti-Sapphire laser is split to pump specimens on two otherwise independent laser scanning microscopes. These microscopes are optimized for the in vivo imaging of highly transparent specimens such as zebrafish larvae. (See publications 85, 88, 91, 92, 93, 97). |
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A closer look at one of the two Stella Coates lab microscopes. A small optical bench situated above the trinocular tube holds beam stearing optics and an X-Y servo galvo pair. Each of the two black boxes at the rear of the scope encloses a two-channel detector consisting of two photomultiplier tubes and a spectral beamsplitter. One detector pair recieves light collected in refelection mode hrough the microscope objective while the other receives light collected via the substage condenser. |
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Squid Rig, 1983. Microelectrode voltage clamp and fiber-coupled polychronmator for arsenazo III measurement of Ca in giant presynaptic terminal. Constructed in collaboration with Milton Charlton and George Augustine at Woods Hole. Enclosed in foil-lined heavy plywood for electrical shielding and sound isolation. Micromanipulators have electric fine advance. (See publications 12-15, 18). |
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Arsenazo III polychromator. Camera lens collimates light from input fiber onto a blazed diffraction grating. Dispersed light is then reflected by a square mirror onto a set of five lens-photodiode housings. (See publication 15). |
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A Woods Hole Blaster Rig, 1983. Dual presynaptic - postsynaptic voltage clamp where local Ca perfusion from a blaster pipette isolates transmitter release to a well-clamped segment of squid preterminal. |
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Blaster pipette on squid stellate ganglion in squid rig, Woods Hole 1983. Micropipettes have been used to inject arsenazo III (red color) into presynatic terminal and fast green (green color) into postsynaptic giant axon. Bulk perfusion with very low Ca flows through chamber from bottom toward top. A large pipette allows normal-Ca solution to contact limited segment of presynaptic terminal. With the addition of a few more micropipettes and a light-sensing fiber tip, the setup was used to measure calcium dependence of transmitter release. |
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"Black Beauty", 1985. Optical bench video microscope. Nose-to-nose objective pair functions as condenser-objective train. Water-cooled lamphouse and newvicon tube imager. (See publication 22). |
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| Early-model Bio-Rad MRC 500 . Stanford 1990. This 'scope has robotic stage for multi-site time-lapse imaging. |
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Falcon Mark 7 laser confocal, 1999. (Smith wearing readers). Field stimuation electronics on shelf. Confocal software by Ziv. |
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Smith lab's first two-photon rig, 1999. Ti-spaphire laser at right rear pumps optical rail inverted microscope. Used for zebrafish embryo spinal cord imaging. |
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An early two-headed two-photon microscope, ~2001. Upright 'scope at front, inverted 'scope at rear. Both 'scope sran over Ziv's Falcon software. |
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The Model 1 microarray reader, 1994. The first Stanford microarray reader supported the earliest years of microarray research here. Medium frame argon/krypton laser pumps stage-scanner built on linear encoder motor stage. A beamsplitter and two PMTs provide for two-color detection. Software by Ziv. (See publication 60). |
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